Oyunda toplam 47 şarkı bulunmaktadır. Şarkılar, biri bonus olmak üzere 7 gruba ayrılmıştır. Bonus şarkılar grubunda 17, diğer gruplarda 5'er şarkı bulunmaktadır. Bu şarkıların 30'u tanınmış gruplardan olmakla beraber, geri kalan 17'si daha az tanınan gruplardandır.
1. Opening Licks
* "I Love Rock & Roll" - Joan Jett & The Blackhearts
* "I Wanna Be Sedated" - The Ramones
* "Thunder Kiss '65" - White Zombie
* "Smoke on the Water" - Deep Purple
* "Infected" - Bad Religion
2. Axe-Grinders
* "Iron Man" - Black Sabbath
* "More Than a Feeling" - Boston
* "You've Got Another Thing Comin'" - Judas Priest
* "Take Me Out" - Franz Ferdinand
* "Sharp Dressed Man" - ZZ Top
3. Thrash and Burn
* "Killer Queen" - Queen
* "Hey You" - The Exies
* "Stellar" - Incubus
* "Heart Full of Black" - Burning Brides
* "Symphony of Destruction" - Megadeth
4. Return of the Shred
* "Ziggy Stardust" - David Bowie
* "Fat Lip" - Sum 41
* "Cochise" - Audioslave
* "Take It Off" - The Donnas
* "Unsung" - Helmet
5. Fret-Burners
* "Spanish Castle Magic" - Jimi Hendrix
* "Higher Ground" - Red Hot Chili Peppers[2]
* "No One Knows" - Queens of the Stone Age
* "Ace Of Spades" - Motörhead
* "Crossroads" - Cream
6. Face-Melters
* "Godzilla" - Blue Öyster Cult
* "Texas Flood" - Stevie Ray Vaughan
* "Frankenstein" - The Edgar Winter Group
* "Cowboys from Hell" - Pantera
* "Bark at the Moon" - Ozzy Osbourne
Bonus
* "Fire It Up" - Black Label Society
* "Cheat on the Church" - Graveyard BBQ
* "Cavemen Rejoice" - The Bags
* "Eureka, I've Found Love" - The Upper Crust
* "All of This" - Shaimus
* "Behind The Mask" - Anarchy Club
* "The Breaking Wheel" - Artillery
* "Callout" - The Acro-brats
* "Decontrol" - Drist
* "Even Rats" - The Slip
* "Farewell Myth" - Made in Mexico
* "Fly on the Wall" - Din
* "Get Ready 2 Rokk" - Freezepop
* "Guitar Hero" - Monkey Steals The Peach
* "Hey" - Honest Bob and the Factory-to-Dealer Incentives
* "Sail Your Ship By" - Count Zero
* "Story of My Love" - The Model Sons
